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IJBT Vol.03 [2004] >
IJBT Vol.03(3) [July 2004] >
| Title: | Kinetic studies of penicillin production during batch and repeated batch in fluidized bed bioreactor with agar immobilized P. chrysogenum cells |
| Authors: | Rani, A Swaroopa
Jetty, Annapurna
Ramakrishna, S V |
| Keywords: | Penicillium chrysogenum
agar
penicillin
batch
repeated batch
fluidized bed reactor |
| Issue Date: | Jul-2004 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A 01 N 63/00; C 12 P 37/00 |
| Abstract: | The fermentation kinetics of penicillin production by Penicillium chrysogenum was carried out at 27°C and pH 6.0. Batch and repeated batch fermentations using agar-immobilized cells in fluidized bed bioreactor were studied for their potential application in production of penicillin from lactose, a fermentable sugar. Kinetics of immobilized cell fermentation, showed the penicillin yield of ~0.0155 g/l, with highest penicillin concentration of ~57.09 mg/l and the high reactor productivity of ~23.8 mg/l h-1. Repeated batch fermentation experiments showed that the immobilized biocatalysts could be recycled effectively for 5 cycles. Penicillin yield was 4-5-fold greater by this method of immobilization, with high productivity as compared to free cells and other immobilization methods. |
| Page(s): | 394-399 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(3) [July 2004]
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