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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(3) [July 2004] >
| Title: | Role of phosphorylase and amylase in accelerated ripening of mutant banana ‘Basrai-10Gy’ |
| Authors: | Surendranathan, K K
Ramaswamy, N K
Pendharkar, M B |
| Keywords: | amylase
banana ripening
mutant
starch metabolism
starch phosphorylase |
| Issue Date: | Jul-2004 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 C 12 N 15/01 |
| Abstract: | ‘Basrai-10Gy’, a mutant developed by  - irradiation of shoot-cultures of the ‘Basrai’ banana, ripened much faster compared to its parent. The biochemical mechanism underlying the faster ripening of the mutant was investigated. Ripening was assessed by determining textural changes in the whole fruit, the peel and the pulp, as well as from the physical changes of ripening. The activities of amylase (E.C.3.2.1.3) and starch phosphorylase (E.C. 2.4.1.21), the enzymes that initiate the catabolism of starch, were comparatively evaluated in the parent and mutant variety. They were correlated with the ripening behaviour of the fruit. An increase in the amylase activity, associated with the climacteric and normal ripening behaviour, was observed in ‘Basrai’ bananas; while, the phosphorylase activity was comparatively insignificant. The mutant variety showed low amylase and very high phosphorylase activities, suggesting the direct availability of sugar phosphate instead of free sugars to accelerate the ripening of ‘Basrai-10Gy’ fruit. |
| Page(s): | 382-387 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(3) [July 2004]
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