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Title: Genetic conservation of plantation crops and spices using cryopreservation
Authors: Chaudhury, Rekha
Malik, S K
Keywords: cryopreservation;seed;embryo;embryonic axes;shoot apices;pollen
Issue Date: Jul-2004
Publisher: CSIR
IPC Code: Int. Cl.7 A 23 B 7/024, 7/055, 9/10
Abstract:  Germplasm conservation in the form of seed is convenient and most cost-effective ex situ conservation method for plant species producing orthodox seeds. Several species of plantation crops and spices produce non-orthodox seeds, which exhibit intermediate or recalcitrant seed storage behaviour. Storage of these species requires the use of in vitro conservation techniques for short- to medium-term conservation and cryopreservation to achieve long-term conservation. Various cryotechniques applicable to 11 genera totaling 16 species [rubber (Hevea brasiliensis Muell.- Arg.), cocoa (Theobroma cacao L.), coconut (Cocos nucifera L.), arecanut (Areca catechu L.), oilpalm (Elaeis guineensis Jacq.), Coffea spp., tea (Camellia sinensis L.(O.) Kuntze), black pepper (Piper nigrum L.), cardamom (Elettaria cardamomum Maton), cashew (Anacardium occidentale L.) and nutmeg (Myristica fragrans Houtt)] are reviewed in the present paper. Physical and physiological factors determining the success or failure of cryopreservation are also discussed.
Page(s): 348-358
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(3) [July 2004]

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