Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5857
Title: Introgression of osmotin gene for creation of resistance against <i style="">Alternaira </i>blight by perturbation of cell cycle machinery
Authors: Taj, Gohar
Kumar, Anil
Bansal, K C
Garg, G K
Keywords: <i style="">Alternaira </i>blight
cell cycle
osmotin
Issue Date: Apr-2004
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A 01 H 4/00, 5/00; C 12 N 5/09, 15/29
Abstract: Pathogenesis related protein, osmotin, when introgressed into <i style="">Brassica juncea</i> plants, provided tolerance to fungal attack. Osmotin is thought to influence the signal transduction pathway. Possible influence of osmotin gene transfer on the cell cycle and cell death pathways was investigated using transformed <i style="">B. juncea</i> calli as model system. It was observed that partially purified <i style="">Alternaria</i> toxin inhibited the growth of non-transformed calli; whereas, transformed calli resisted the effect of toxin, which appears to counteract the inhibitory effects of phytotoxins. The toxin also initiates the programmed cell death (PCD) in the calli. The toxin increased the expression of P<sup>53</sup> and caspase-like proteins in the non-transformed calli and suppressed the expression of cyclin B and CDC proteins. It was further supported by the fragmentation of DNA in response to toxin treatment. However, the transformed calli, when challenged with toxin, suppressed the expression of P<sup>53</sup>, while caspase 1 was not affected. On the basis these results, it can be concluded that osmotin tampers the P<sup>53</sup>-mediated PCD pathway; which, although, could not provide the resistance but delayed the appearance of disease symptoms.
Description: 291-298
URI: http://hdl.handle.net/123456789/5857
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(2) [April 2004]

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