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Title: A strategy to develop disabled infectious single-cycle (DISC) foot and mouth disease virus by 3B3 gene deletion using the infective cDNA copy of the genome
Authors: Sarkar, Swaroop
Suryanarayana, V V S
Sekar, S Chandra
Shankar, S R Madan
Reddy, G R
Dechamma, H J
Keywords: Disabled infectious single cycle;3B3 gene deleted replicon;BHK cells expressing 3B3;FMDV virulence;attenuation.
Issue Date: Jan-2021
Publisher: NIScPR-CSIR, India
Abstract: Disabled infectious single-cycle (DISC) virus is in between attenuated and inactivated. When used as a vaccine DISC virus behaves like inactivated virus as it cannot further multiply in the vaccinated individual after one cycle of replication. When infected to permissive cells expressing virus specific protein which has been deleted from viral genome, the virus replicates normally. The development of DISC virus involves the deletion of an open reading frame (ORF) coding for a key protein involved in the viral replication or viral capsid formation. Such virus, when injected in animals, can complete only one round of replication without producing a progeny virus. Here we report similar strategy followed for the production DISC foot and mouth disease virus (FMDV). We have selected 3B3 protein gene that expresses 3 copies of virus specific genome-linked protein needed for virus replication and deleted from the FMDV replicon carrying FMDV serotype Asia 1 backbone and inserted the same into baby hamster kidney 21 (BHK-21) cell genome. Upon transfection of the genetically modified BHK cells with RNA copy of the genetically modified cDNA, replication of the virus started in these cells. The DISC virus so developed can be a potent vaccine candidate for achieving robust and long duration of immune response against FMDV infection in bovine. The approach also took care in the development of serotype specific DISC viruses using single FMDV Asia 1 replicon. Vaccines based on DISC viruses may be superior in terms of immune response as compared to inactivated vaccines.
Page(s): 35-42
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.20(1) [January 2021]

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