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Title: In vitro cloning of Cajanus cajan var. Bahar through prolific shoot bud differentiation in leaf segments and production of fertile plants
Authors: Jain, M
Chaturvedi, H C
Keywords: Bahar;Cajanus cajan;fertile plants;interveinal leaf lamina;in vitro cloning;shoot bud differentiation
Issue Date: Apr-2004
Publisher: CSIR
IPC Code: Int. Cl.7 A 01 H 4/00, 5/00
Abstract: Prolific differentiation of shoot buds was induced in the interveinal leaf lamina explants (5 mm ´ 2 mm) excised from first pair of leaves of aseptically grown plants of Cajanus cajan var. Bahar. An explant cultured on filter paper bridge in a nutrient liquid medium supplemented with 0.25 mg l-1 each of BAP and Kn along with 0.05 mg l-1 IAA and 40 mg l-1 AdS, produced an av. 50 shoot buds within 45 days, after subjected to a pulse treatment comprising 2 mg l-1 each of BAP and Kn with 1 mg l-1 IAA for three days given under the same culture conditions. Development of normal shoots and simultaneous differentiation of new shoot buds were obtained by subculture of explants with differentiated shoot buds on agarified nutrient medium mainly in the presence of 0.01 mg l-1 each of BAP and IAA along with 10 mg l-1 CCC, where even more than 100 shoot buds were formed in the best cultures. Developed shoots rooted 100% in 0.25 mg l-1 of any of the three auxins used, viz, IAA, IBA and NAA, while the clonal plantlets gave 90% transplant success. In vitro-raised plants, when transplanted to field in mid-October, grew normally and produced viable seeds by late-March. The seeds, so produced, germinated 100% in vitro and gave rise to normally growing plants in soil. The protocol for cloning of this commercially important variety also offers an excellent system for its genetic transformation in having certain outstanding features, which include simultaneous differentiation of a great number of shoot meristems in a uniform leaf tissue explant and development of virtually all of them into normal fertile plants.
Page(s): 258-262
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(2) [April 2004]

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