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|Title:||Production and identification of intricate bioactive oligosaccharides from Nyctanthesarbor-tristis leaves by a combination of enzymatic, HPAEC and MALDI-TOF-MS techniques|
|Keywords:||N. arbor-tristis leaves;hemicelluloses;oligosaccharides;HPAEC;MALDI-TOF-MS|
|Abstract:||Non-digestible oligosaccharides are associated with positive health outcomes; however, hemicellulosic oligosaccharides of N. arbor-tristis have not been studied. Herein, we report production of hemicellulosic oligosaccharides from its leaves and their identification. A series of complex neutral oligosaccharides (1-16), produced by endo-(1,4)--D-glucanase digestion of the alkali extracted xyloglucan, have been identified by chemical, HPAE chromatographic and MALDI-mass spectrometric techniques. Additionally, xylan enriched hemicellulose-A fraction upon endo-(1,4)--D-xylanase digestion yield several acidic and neutral xylo-oligosaccharides (17-24), each containing a backbone of -(1,4)-D-Xylp units substituted at position 2 with a single stub of D-Xylp or 4-O-Me-D-GlcpA residues. The structural diversity and high water solubility of these enzyme-generated oligosaccharides suggested promising health implications.|
|Appears in Collections:||IJC-B Vol.60B(11) [November 2021]|
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|IJC (Section B), 60B, 1471-1477 (Nov,2021).pdf||3.96 MB||Adobe PDF||View/Open|
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