Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/58500
Title: Bioactivity guided isolation and characterization of antimicrobial principle from Hydrocotyle javanica Thunb.
Authors: Sood, Kaushal
Yadav, R N S
Keywords: Antimicrobial activity;Bioautography;Cytotoxicity;Hydrocotyle javanica;Structure
Issue Date: Oct-2021
Publisher: CSIR-NIScPR
IPC Code: Int Cl.21: A61K 36/25, A61K 45/06
Abstract: Hydrocotyle javanica Thunb. (Family Araliaceae) has been used in traditional herbal medicine as a substitute of Centella asiatica (L.) Urban. Although, the latter has been studied extensively and various biologically active constituents have been reported, the former has not been studied for its active constituents. The current study followed activity guided isolation and identification of the fraction with antimicrobial activity from H. javanica. Plant extracts were prepared by extracting successively with petroleum ether, ethyl acetate and ethanol. Antimicrobial activity was evaluated using gram positive and gram negative bacteria and Candida albicans. Bioactivity-guided isolation was carried out by preparative thin layer chromatography and bioautography. Cytotoxicity of the most active antimicrobial principle was evaluated against Human embryonic kidney (HEK293) and Adenocarcinomic Human Alveolar Basal Epithelial (A549) cell lines. The tentative structure of the most active antimicrobial principle was constructed from the data obtained from FTIR, MS, H-NMR and C-NMR.
Page(s): 913-919
ISSN: 0975-1068 (Online); 0972-5938 (Print)
Appears in Collections:IJTK Vol.20(4) [October 2021]

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