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Title: Cloning of adult trrees of Jamun (Syzygium cuminii)
Authors: Rathore, Vinod
Shekhawat, N S
Singh, R P
Rathore, J S
Dagla, H R
Keywords: Cloning;Jamun;mature tree;nodal shoots;Syzygium cuminii
Issue Date: Apr-2004
Publisher: CSIR
IPC Code: Int.Cl.7 A 01 H 4/00, 5/00
Abstract: For cloning of adult tree(s) of Jamun (Syzygium cuminii), explants were harvested from rejuvenated shoot sprouts produced by lopped tree(s). Multiple shoots regenerated by activation of axillary meristems of the explants on MS + 9.0 µM BAP + growth additives. Shoots were proliferated in culture by (i) repeated transfer of original explants, and (ii) subculture of shoots on amended medium with combination of BAP (4.50 µM) + kinetin (2.25 µM). The cloned shoots were rooted (i) in vitro on half-strength MS + 0.1% activated charcoal supplemented with IBA (10 µM) or NAA (15.0 µM), and (b) ex vitro by pulse treatment with 2.50 mM of IBA. The shoots root 100% under ex vitro conditions. The cloned treelets were hardened and pot transferred. The ex vitro rooting of cloned shoots was highly effective; it saved time and resources, and can be used for cloning of Jamun.
Page(s): 241-245
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(2) [April 2004]

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