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Title: Cloning of adult trrees of Jamun (<i style="">Syzygium cuminii</i>)
Authors: Rathore, Vinod
Shekhawat, N S
Singh, R P
Rathore, J S
Dagla, H R
Keywords: Cloning
mature tree
nodal shoots
<i style="">Syzygium cuminii</i>
Issue Date: Apr-2004
Publisher: CSIR
Series/Report no.: Int.Cl.<sup>7</sup> A 01 H 4/00, 5/00
Abstract: For cloning of adult tree(s) of Jamun (<i style="">Syzygium cuminii</i>), explants were harvested from rejuvenated shoot sprouts produced by lopped tree(s). Multiple shoots regenerated by activation of axillary meristems of the explants on MS + 9.0 µ<i style="">M</i> BAP + growth additives. Shoots were proliferated in culture by (i) repeated transfer of original explants, and (ii) subculture of shoots on amended medium with combination of BAP (4.50 µ<i style="">M</i>) + kinetin (2.25 µ<i style="">M</i>). The cloned shoots were rooted (i) <i style="">in vitro</i> on half-strength MS + 0.1% activated charcoal supplemented with IBA (10 µ<i style="">M</i>) or NAA (15.0 µ<i style="">M</i>), and (b) <i style="">ex vitro</i> by pulse treatment with 2.50 m<i style="">M</i> of IBA. The shoots root 100% under <i style="">ex vitro</i> conditions. The cloned treelets were hardened and pot transferred. The <i style="">ex vitro</i> rooting of cloned shoots was highly effective; it saved time and resources, and can be used for cloning of Jamun.
Description: 241-245
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(2) [April 2004]

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