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Title: Micropropagation of an adult tree-Wrightia tinctoria
Authors: Purohit, S D
Kukda, G
Keywords: clonal propagation
Wrightia tinctoria
tissue culture
Issue Date: Apr-2004
Publisher: CSIR
IPC CodeInt Cl.7 A 0 1 H 4/00, 5/00
Abstract: Multiple shoots were induced in vitro from nodal shoot segments of a 30-yr-old plus tree having enhanced axillary branching. Initiation of culture was strongly influenced by the nature of explant and the season of harvesting. Nodal segments (91%) from young lateral branches produced an average of 5 shoots per node in 3 weeks on agar solidified MS medium supplemented with 2.0 mg l-1 benzylaminopurine (BAP). After establishment of cultures and initiation of shoot buds, a cluster of shoots including mother explant, was transferred to medium containing a lower concentration of BAP (1.0 mg l-1). A three-fold rate of shoot multiplication during every subculture of 3 weeks was achieved. Nodal segments from in vitro raised shoots were also used to initiate a new culture cycle. The shoots could be multiplied for at least 24 months without loss of vigour. The shoots (71%) were successfully rooted when their lower ends were dipped in pre-autoclaved indole-3-butyric acid solution (100 mg l-1) for 10 min followed by their implantation on modified MS medium (major salts reduced to 1/4 strength) containing 200 mg l-1 activated charcoal. Out of 2000 plants, 1600 (80%) were successfully hardened under greenhouse conditions and more than 200 have been planted out in soil where they are growing well.
Page(s): 216-220
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(2) [April 2004]

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