NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(2) [April 2004] >


Title: Studies on in vitro propagation of Himalayan cedar (Cedrus deodara) using zygotic embryos and stem segments
Authors: Tamta, Sushma
Palni, L M S
Keywords: Cedrus deodara
conifer
deodar
micropropagation
inverted embryo technique
stem segments
rooting
Issue Date: Apr-2004
Publisher: CSIR
IPC CodeInt Cl.7 A 01 H 4/00, 7/00
Abstract: Adventitious bud formation was examined in zygotic embryos (decoated seeds) of Cedrus deodara, planted in the normal position, using four different media variously supplemented with or without plant growth regulators (PGRs). Following incubation for 7, 14, 21 and 28 days on PGR containing media, the embryos were transferred to respective PGR-free basal media. While embryo swelling was observed in all cases, normal germination was observed only in embryos cultured initially in PGR-free media. Best response, in terms of bud formation (93%), was observed in embryos first cultured on LP medium containing BA and Kinetin (1.0 M each) for 28 days and then transferred to LP medium without any PGR. While the mean number of shoots formed per cultured embryo was also highest (34%) in this combination, these shoots eventually dried when excised and sub-cultured for further multiplication. Success was, however, obtained when the embryos were initially planted in an inverted position on LP medium containing 20.0 M BA for 10 days, and then shifted, again in the inverted position, on the same medium without BA. Although the mean number of shoots formed was low (only 2.5 shoots per embryo), these could be multiplied and elongated. The microshoots (2.0-3.0 cm) thus obtained could be rooted (100%) using ½ strength, PGR-free, LP medium, hardened and established in pots. Besides excised embryos, stem segments were also used as explants. While only a quarter of explants could be established in culture, in contamination-free condition, good sprouting response (3-4 buds per segment) was obtained in certain PGR combinations. The sprouted buds developed into shoots on LP medium containing 5.0 M BA; further subculturing of excised shoots for multiplication, however, resulted in drying up of shoots. Further work is required to overcome this difficulty.
Page(s): 209-215
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(2) [April 2004]

Files in This Item:

File Description SizeFormat
IJBT 3(2) 209-215.pdf207.98 kBAdobe PDFView/Open
 Current Page Visits: 747 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 622539 since 06-Feb-2009  Last updated on 13-Nov-2014Webmaster: nopr@niscair.res.in