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Title: Isolated microspore culture of Brassica: An experimental tool for developmental studies and crop improvement
Authors: Babbar, Shashi B
Agarwal, Pradeep K
Sahay, Shalini
Bhojwani, Sant S
Keywords: Brassica
isolated microspore culture
anther culture
crop improvement
genetic transformation
germplasm storage
Issue Date: Apr-2004
Publisher: CSIR
IPC CodeInt. Cl.7 A 01 H 1/08, 4/00, 5/00; C 12 N 15/00, 15/01
Abstract: The technique of isolated microspore culture for the production of haploid plants is well standardized for many species of Brassica, some of which are important oilseed or vegetable crops. It offers an excellent experimental system for improve-ment of Brassica crops and study of cell differentiation during shift from gametophytic to sporophytic development. The haploids raised from microspores are of tremendous use in genetic improvement of brassicas through breeding, mutant induction and selection, and genetic engineering. The present review describes current status of the technology, the factors influencing the success of microspore culture, structural, biochemical and molecular changes occurring in the microspores triggered towards sporophytic development, and the progress made in practical applications of the microspore-derived plants.
Page(s): 185-202
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(2) [April 2004]

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