Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5844
Title: Isolated microspore culture of <i style="">Brassica</i>: An experimental tool for developmental studies and crop improvement
Authors: Babbar, Shashi B
Agarwal, Pradeep K
Sahay, Shalini
Bhojwani, Sant S
Keywords: <i style="">Brassica</i>
isolated microspore culture
anther culture
haploids
crop improvement
diplodization
mutants
genetic transformation
germplasm storage
Issue Date: Apr-2004
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A 01 H 1/08, 4/00, 5/00; C 12 N 15/00, 15/01
Abstract: The technique of isolated microspore culture for the production of haploid plants is well standardized for many species of <i style="">Brassica,</i> some of which are important oilseed or vegetable crops. It offers an excellent experimental system for improve-ment of <i style="">Brassica </i>crops and study of cell differentiation during shift from gametophytic to sporophytic development. The haploids raised from microspores are of tremendous use in genetic improvement of brassicas through breeding, mutant induction and selection, and genetic engineering. The present review describes current status of the technology, the factors influencing the success of microspore culture, structural, biochemical and molecular changes occurring in the microspores triggered towards sporophytic development, and the progress made in practical applications of the microspore-derived plants.
Description: 185-202
URI: http://hdl.handle.net/123456789/5844
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(2) [April 2004]

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