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Title:  Identification of in vitro responsive immature embryo size for plant regeneration in Sudan grass (Sorghum sudanenses Piper)
Authors: Gupta, Sanjay
Khanna, V K
Singh, Rameshwar
Garg, G K
Keywords: sorghum;Sorghum sudanenses;immature embryos;immature embryo size;callus induction;plant regeneration
Issue Date: Jan-2004
Publisher: CSIR
Abstract:  Immature embryos of various sizes from six genotypes of sudan grass (Sorghum sudanenses Piper) were cultured to identify the best in vitro responsive embryo size. The immature embryos size influenced callus formation (days to callus initiation, callus induction frequency, callus growth) and plant regeneration (shoot induction frequency, shoots per callus) parameters. Immature embryos (0.7-1.5 mm) were quicker to initiate callus, induced fastly growing callus in higher frequency and regenerated shoots more frequently and intensely than immature embryos (1.6-2.5 mm) because of the initiation of callus from rapidly dividing scuttelar cells. Frequent germination and initiation of non-embryogenic callus from plumule or radicle were the main reasons for poor callus induction and plant regeneration responses from 1.6-2.5 mm size immature embryos. Thus immature embryos of 0.7-1.5 mm size may be used for embryogenic culture initiation and plant regeneration.
Page(s): 124-127
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(1) [January 2004]

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