NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(1) [January 2004] >


Title: Factors enhancing somatic embryogenesis and plant regeneration in sugarcane (Saccharum officinarum L.)
Authors: Gill, Navdeep K
Gill, Raman
Gosal, S S
Keywords: somatic embryogenesis
plant regeneration
maltose
proline
dessication
auxin
sugarcane
Issue Date: Jan-2004
Publisher: CSIR
Abstract:  Factors affecting somatic embryogenesis and subsequent plant regeneration in vitro in sugarcane cultures were highly genotype specific. They were affected by medium constitution, auxins, sugars, amino acids, growth retardants, and desiccation, etc. Supplementation of the control medium independently with maltose (30g/l), proline (560mg/l), abscissic acid [ABA] (2mg/l) and reduced auxin ie 2,4-Dichlorophenoxyacetic acid [2,4-D] (3mg/l) enhanced somatic embryogenesis considerably. Similarly, higher shoot regeneration was achieved on a medium containing only cytokinin ie 6-Benzylaminopurine [BAP] (0.5mg/l). Addition of ABA (2mg/l), proline (560mg/l) and dessicating conditions, created by increasing agar concentration, also increased shoot regeneration. Rooting was induced completely in the rooting medium containing only -naphthalene acetic acid [NAA] (5mg/l) rather than a combination of indole butyric acid [IBA] (2mg/l) and NAA (3mg/l) both accompanied by high sugar ie sucrose (7%).
Page(s): 119-123
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(1) [January 2004]

Files in This Item:

File Description SizeFormat
IJBT 3(1) 119-123.pdf194.35 kBAdobe PDFView/Open
 Current Page Visits: 612 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 558011 since 06-Feb-2009  Last updated on 15-Jul-2014Webmaster: nopr@niscair.res.in