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Title: Factors enhancing somatic embryogenesis and plant regeneration in sugarcane (<i style="">Saccharum officinarum</i> L.)
Authors: Gill, Navdeep K
Gill, Raman
Gosal, S S
Keywords: somatic embryogenesis
plant regeneration
Issue Date: Jan-2004
Publisher: CSIR
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="City"><smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place" downloadurl=""> Factors affecting somatic embryogenesis and subsequent plant regeneration <i style="">in vitro</i> in sugarcane cultures were highly genotype specific. They were affected by medium constitution, auxins, sugars, amino acids, growth retardants, and desiccation, etc. Supplementation of the control medium independently with maltose (30g/l), proline (560mg/l), abscissic acid [ABA] (2mg/l) and reduced auxin ie 2,4-Dichlorophenoxyacetic acid [2,4-D] (3mg/l) enhanced somatic embryogenesis considerably. Similarly, higher shoot regeneration was achieved on a medium containing only cytokinin ie 6-Benzylaminopurine [BAP] (0.5mg/l). Addition of ABA (2mg/l), proline (560mg/l) and dessicating conditions, created by increasing agar concentration, also increased shoot regeneration. Rooting was induced completely in the rooting medium containing only <img src='/image/spc_char/alpha.gif'> -naphthalene acetic acid [NAA] (5mg/l) rather than a combination of indole butyric acid [IBA] (2mg/l) and NAA (3mg/l) both accompanied by high sugar ie sucrose (7%). </smarttagtype></smarttagtype>
Description: 119-123
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(1) [January 2004]

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