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Title: Factors enhancing somatic embryogenesis and plant regeneration in sugarcane (Saccharum officinarum L.)
Authors: Gill, Navdeep K
Gill, Raman
Gosal, S S
Keywords: somatic embryogenesis
plant regeneration
Issue Date: Jan-2004
Publisher: CSIR
Abstract:  Factors affecting somatic embryogenesis and subsequent plant regeneration in vitro in sugarcane cultures were highly genotype specific. They were affected by medium constitution, auxins, sugars, amino acids, growth retardants, and desiccation, etc. Supplementation of the control medium independently with maltose (30g/l), proline (560mg/l), abscissic acid [ABA] (2mg/l) and reduced auxin ie 2,4-Dichlorophenoxyacetic acid [2,4-D] (3mg/l) enhanced somatic embryogenesis considerably. Similarly, higher shoot regeneration was achieved on a medium containing only cytokinin ie 6-Benzylaminopurine [BAP] (0.5mg/l). Addition of ABA (2mg/l), proline (560mg/l) and dessicating conditions, created by increasing agar concentration, also increased shoot regeneration. Rooting was induced completely in the rooting medium containing only -naphthalene acetic acid [NAA] (5mg/l) rather than a combination of indole butyric acid [IBA] (2mg/l) and NAA (3mg/l) both accompanied by high sugar ie sucrose (7%).
Page(s): 119-123
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(1) [January 2004]

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