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Title: Efficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk
Authors: Giridhar, P
Ravishankar, G A
Keywords: axillary bud
bulbous shoot buds
gibberilic acid
Issue Date: Jan-2004
Publisher: CSIR
Abstract: Vanilla planifolia multiplication through tissue culture was worked out with new leads in elucidation of synergistic activity of zeatin, synthetic cytokinin thidiazuron (TDZ) and coconut milk (CM) as well as N6-benzyladenine(BA) . Multiple shoots were developed from axillary bud explants using Murashige and Skoog (MS) medium supplemented with zeatin, BA+zeatin , TDZ and TDZ+ coconut milk (CM). The nature of explant and the method of explant inoculation onto the medium influence not only multiple shoot production, but also bulbous shoot buds (BS) formation. Zeatin supported the growth of mostly single shoots, whereas formation of BS was induced in zeatin and BA combination and also in media supplemented with TDZ+10% CM. Subsequent transfer of these BS onto shoot proliferation medium supplemented with BA (8.87 M) or -naphthalene acetic acid (NAA) (2.69 M) resulted in multiple shoot proliferation 17 ± 2.5 shoots and 30 ± 2.1 shoots respectively per explant. The multiple shoots so obtained were transferred to Nitsch medium (N69) containing BA (2.22 M) and gibberellic acid (GA3 ) (0.029 M) and also onto simultaneous shoot multiplication and root forming medium for further growth. For the first time the influence of zeatin, TDZ and coconut milk on shoot multiplication was studied. This protocol is effective in producing micropropagated vanilla plants with successful hardening and field transfer.
Page(s): 113-118
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(1) [January 2004]

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