Please use this identifier to cite or link to this item:
Title: High frequency shoot regeneration from Phyllanthus amarus Schum. & Thonn.
Authors: Ghanti, Kiran S
Govindaraju, B
Venugopal, R B
Rao, S Ramgopal
Kaviraj, C P
Jabeen, F T Z
Barad, Arvind
Rao, Srinath
Keywords: Phyllanthus amarus;micropropagation;multiple shoots;rhizogenesis
Issue Date: Jan-2004
Publisher: CSIR
Abstract: Shoot tip, nodal and internodal segments of Phyllanthus amarus Schum. & Thonn. were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyl amino purine (BAP) and kinetin (Kn) with 15% coconut milk. All the explants responded for regeneration. High frequency (100%) and maximum number of shoots (18.30 ± 0.47) were recorded on medium containing BAP (0.5 mg/l) in shoot tip culture. In vitro shoots were excised from shoot clumps and transferred to rooting medium containing different concentrations of indole butyric acid (IBA) or naphthalene acetic acid (NAA). Maximum number of roots was induced in medium containing IBA (0.5 mg/l). Rooted plantlets were hardened on MS basal liquid medium and subsequently in sterile soil + vermiculite (1:1). Plantlets thus developed were successfully established and finally transferred to greenhouse. The survival rate of the plantlets in the field was found to be very high (85%).
Page(s): 103-107
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(1) [January 2004]

Files in This Item:
File Description SizeFormat 
IJBT 3(1) 103-107.pdf199.85 kBAdobe PDFView/Open

Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.