Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5835
Title: High frequency shoot regeneration from <i style="">Phyllanthus amarus</i> Schum. & Thonn.
Authors: Ghanti, Kiran S
Govindaraju, B
Venugopal, R B
Rao, S Ramgopal
Kaviraj, C P
Jabeen, F T Z
Barad, Arvind
Rao, Srinath
Keywords: <i style="">Phyllanthus amarus</i>
micropropagation
multiple shoots
rhizogenesis
Issue Date: Jan-2004
Publisher: CSIR
Abstract: Shoot tip, nodal and internodal segments of <i style="">Phyllanthus amarus </i>Schum. & Thonn. were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyl amino purine (BAP) and kinetin (Kn) with 15% coconut milk. All the explants responded for regeneration. High frequency (100%) and maximum number of shoots (18.30 ± 0.47) were recorded on medium containing BAP (0.5 mg/l) in shoot tip culture. <i style="">In vitro</i> shoots were excised from shoot clumps and transferred to rooting medium containing different concentrations of indole butyric acid (IBA) or naphthalene acetic acid (NAA). Maximum number of roots was induced in medium containing IBA (0.5 mg/l). Rooted plantlets were hardened on MS basal liquid medium and subsequently in sterile soil + vermiculite (1:1). Plantlets thus developed were successfully established and finally transferred to greenhouse. The survival rate of the plantlets in the field was found to be very high (85%).
Description: 103-107
URI: http://hdl.handle.net/123456789/5835
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(1) [January 2004]

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