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Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(1) [January 2004] >
| Title: | Transformation of oilseed mustard Brassica juncea (L.) Czern & Coss cv. Pusajaikisan with snowdrop lectin gene |
| Authors: | Sharma, Manju
Sahni, Rohini
Kansal, Rekha
Koundal, K R |
| Keywords: | Brassica juncea
Agrobacterium tumefaciens
snowdrop lectin gene
stem segments
transformation |
| Issue Date: | Jan-2004 |
| Publisher: | CSIR |
| Abstract: | An efficient protocol has been developed to transfer snowdrop lectin gene (gna) to Brassica juncea (L.) Czern & Coss cv. Pusajaikisan through Agrobacterium tumefaciens GV 2260 mediated transformation. High frequency regeneration of transformed plantlets has been achieved by using stem segments as explants. Analysis of the putative transformants by PCR amplification and Southern hybridization of genomic DNA showed the successful integration of the transgene in the nuclear genome. The transgenic plants will be further tested for bioassay in future. |
| Page(s): | 97-102 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(1) [January 2004]
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