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Title: Transformation of oilseed mustard Brassica juncea (L.) Czern & Coss cv. Pusajaikisan with snowdrop lectin gene
Authors: Sharma, Manju
Sahni, Rohini
Kansal, Rekha
Koundal, K R
Keywords: Brassica juncea;Agrobacterium tumefaciens;snowdrop lectin gene;stem segments;transformation
Issue Date: Jan-2004
Publisher: CSIR
Abstract: An efficient protocol has been developed to transfer snowdrop lectin gene (gna) to Brassica juncea (L.) Czern & Coss cv. Pusajaikisan through Agrobacterium tumefaciens GV 2260 mediated transformation. High frequency regeneration of transformed plantlets has been achieved by using stem segments as explants. Analysis of the putative transformants by PCR amplification and Southern hybridization of genomic DNA showed the successful integration of the transgene in the nuclear genome. The transgenic plants will be further tested for bioassay in future.
Page(s): 97-102
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(1) [January 2004]

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