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http://nopr.niscair.res.in/handle/123456789/5834| Title: | Transformation of oilseed mustard Brassica juncea (L.) Czern & Coss cv. Pusajaikisan with snowdrop lectin gene |
| Authors: | Sharma, Manju Sahni, Rohini Kansal, Rekha Koundal, K R |
| Keywords: | Brassica juncea;Agrobacterium tumefaciens;snowdrop lectin gene;stem segments;transformation |
| Issue Date: | Jan-2004 |
| Publisher: | CSIR |
| Abstract: | An efficient protocol has been developed to transfer snowdrop lectin gene (gna) to Brassica juncea (L.) Czern & Coss cv. Pusajaikisan through Agrobacterium tumefaciens GV 2260 mediated transformation. High frequency regeneration of transformed plantlets has been achieved by using stem segments as explants. Analysis of the putative transformants by PCR amplification and Southern hybridization of genomic DNA showed the successful integration of the transgene in the nuclear genome. The transgenic plants will be further tested for bioassay in future. |
| Page(s): | 97-102 |
| URI: | http://hdl.handle.net/123456789/5834 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.03(1) [January 2004] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 3(1) 97-102.pdf | 558.37 kB | Adobe PDF | View/Open |
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