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Title: Dot-blot assay using reverse-probe genomic hybridization of repetitive DNA for environmental monitoring of fluorescent Pseudomonads
Authors: Goel, Reeta
Kumar, Praveen
Sinha, Rashmi
Ambwani, Sonu
Keywords: Pseudomonas fluorescens
reverse-probe genomic hybridization
dot-blot assay
soil DNA
repetitive sequences
Issue Date: Jan-2004
Publisher: CSIR
Abstract: The ability of fluorescent pseudomonads to produce antimicrobial metabolites, siderophores and plant hormones makes them an important biocontrol agent as well as plant growth promotary rhizobacteria. For an efficient and potential use as bioinoculant, their quick, specific and sensitive monitoring in environmental sample is essential. A quick detection protocol has been proposed for Pseudomonas fluorescens in soil samples. The approach involved shotgun cloning of restricted genomic DNA with subsequent selection of cloned repetitive DNA by reverse-probe genomic hybridization. In which the plasmid DNA clones were probed with labelled genomic DNA. Further, plasmid DNA with repetitive sequences was used as probe for highly sensitive and specific detection of the soil rhizobacteria.
Page(s): 82-85
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(1) [January 2004]

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