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Title: Induction and properties of (1→3)--D-glucanase from Aureobasidium pullulans
Authors: Dake, M S
Jadhav, J P
Patil, N B
Keywords: Aureobasidium pullulans
induction
glucanase
purification
extracellular enzymes
-glucan
Issue Date: Jan-2004
Publisher: CSIR
Abstract: The structure determination of polysaccharides using specific enzyme(s) as a tool has been of great importance. Such an enzyme, (1→3)--D-glucanase was induced in Aureobasidium pullulans by yeast -glucan as a potent inducer. The formation of enzyme was, however, repressed by glucose. The purified enzyme has molecular weight 230 kDa, optimum pH 5.5 and optimum temperature 60°C. The enzyme hydrolyzes laminarin and yeast -glucan. The km value obtained for laminarin was 0.17%. The analysis of the hydrolyzed product demonstrates that the enzyme acts on (1,3) linkages from the non-reducing end(s).
Page(s): 58-64
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(1) [January 2004]

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