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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(1) [January 2004] >
| Title: | Induction and properties of (1→3)- -D-glucanase from Aureobasidium pullulans |
| Authors: | Dake, M S
Jadhav, J P
Patil, N B |
| Keywords: | Aureobasidium pullulans
induction
glucanase
purification
extracellular enzymes
-glucan |
| Issue Date: | Jan-2004 |
| Publisher: | CSIR |
| Abstract: | The structure determination of polysaccharides using specific enzyme(s) as a tool has been of great importance. Such an enzyme, (1→3)--D-glucanase was induced in Aureobasidium pullulans by yeast -glucan as a potent inducer. The formation of enzyme was, however, repressed by glucose. The purified enzyme has molecular weight 230 kDa, optimum pH 5.5 and optimum temperature 60°C. The enzyme hydrolyzes laminarin and yeast -glucan. The km value obtained for laminarin was 0.17%. The analysis of the hydrolyzed product demonstrates that the enzyme acts on (1,3) linkages from the non-reducing end(s). |
| Page(s): | 58-64 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.03(1) [January 2004]
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