Please use this identifier to cite or link to this item:
http://nopr.niscair.res.in/handle/123456789/5827| Title: | Induction and properties of (1→3)- -D-glucanase from Aureobasidium pullulans |
| Authors: | Dake, M S Jadhav, J P Patil, N B |
| Keywords: | Aureobasidium pullulans;induction;glucanase;purification;extracellular enzymes;-glucan |
| Issue Date: | Jan-2004 |
| Publisher: | CSIR |
| Abstract: | The structure determination of polysaccharides using specific enzyme(s) as a tool has been of great importance. Such an enzyme, (1→3)--D-glucanase was induced in Aureobasidium pullulans by yeast -glucan as a potent inducer. The formation of enzyme was, however, repressed by glucose. The purified enzyme has molecular weight 230 kDa, optimum pH 5.5 and optimum temperature 60°C. The enzyme hydrolyzes laminarin and yeast -glucan. The km value obtained for laminarin was 0.17%. The analysis of the hydrolyzed product demonstrates that the enzyme acts on (1,3) linkages from the non-reducing end(s). |
| Page(s): | 58-64 |
| URI: | http://hdl.handle.net/123456789/5827 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Appears in Collections: | IJBT Vol.03(1) [January 2004] |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJBT 3(1) 58-64.pdf | 262.28 kB | Adobe PDF | View/Open |
Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.