NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.03 [2004] >
IJBT Vol.03(1) [January 2004] >

Title: Quantification of urinary oxalate by immobilized oxalate oxidase of forage sorghum leaf
Authors: Kalra, Vijay
Pundir, C S
Keywords: oxalate
oxalate oxidase
forage sorghum
alkylamine glass
Issue Date: Jan-2004
Publisher: CSIR
Abstract: A partially purified oxalate oxidase from leaves of 10-day-old seedlings of forage sorghum was immobilized covalently onto alkylamine glass beads affixed on inner base of a glass beaker. The enzyme retained 5.17% of its initial activity with a conjugation yield of 182 mg/g support. After immobilization, the enzyme showed an increase in its optimum pH and Km for oxalate but slight decrease in incubation temperature and time for maximum activity as compared to free enzyme. The glass beaker bound enzyme was employed for determination of urinary oxalate. The urinary oxalate in apparently healthy persons, as measured by the glass beaker, was found to be in the range of 12.5 to 29.7 mg/l (mean 20.9 mg/l) for females and 25.7 to 45.4 mg/l urine (mean 37.2 mg/l) for males. The glass beaker provided 70 reuses of immobilized enzyme with ease in handling.
Page(s): 52-57
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(1) [January 2004]

Files in This Item:

File Description SizeFormat
IJBT 3(1) 52-57.pdf183.7 kBAdobe PDFView/Open
 Current Page Visits: 872 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 170576 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: