Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5823
Title: Detection of virulence in Indian isolates of Salmonella by polymerase chain reaction
Authors: Saxena, M K
Singh, V P
Lackchura, B D
Saxena, Anjani
Sharma, Bhaskar
Keywords: Salmonella;serovars;virulence;plasmid;polymerase chain reaction (PCR);Vir gene
Issue Date: Jan-2004
Publisher: CSIR
Abstract:  Virulence in Salmonella is reported to be caused by large plasmids of 60-100 kb. In the present study, 24 isolates of four Salmonella serovars (S. Dublin, S. Abortusequi, S. Bareilly and S. Choleraesuis) were studied for their in vivo virulence, plasmid profiling and virulence (Vir) gene detection by PCR. Nineteen isolates were found to be virulent inducing different degrees of mortality in mice. In 5 non-virulent isolates, the large plasmid was absent and there was also no amplification of Vir gene by PCR. It clearly indicates a positive correlation between virulence of the organism and the presence of plasmid and amplification of Vir gene.
Page(s): 37-40
URI: http://hdl.handle.net/123456789/5823
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(1) [January 2004]

Files in This Item:
File Description SizeFormat 
IJBT 3(1) 37-40.pdf462.41 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.