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IJBT Vol.03(1) [January 2004] >


Title: Detection of virulence in Indian isolates of Salmonella by polymerase chain reaction
Authors: Saxena, M K
Singh, V P
Lackchura, B D
Saxena, Anjani
Sharma, Bhaskar
Keywords: Salmonella
serovars
virulence
plasmid
polymerase chain reaction (PCR)
Vir gene
Issue Date: Jan-2004
Publisher: CSIR
Abstract:  Virulence in Salmonella is reported to be caused by large plasmids of 60-100 kb. In the present study, 24 isolates of four Salmonella serovars (S. Dublin, S. Abortusequi, S. Bareilly and S. Choleraesuis) were studied for their in vivo virulence, plasmid profiling and virulence (Vir) gene detection by PCR. Nineteen isolates were found to be virulent inducing different degrees of mortality in mice. In 5 non-virulent isolates, the large plasmid was absent and there was also no amplification of Vir gene by PCR. It clearly indicates a positive correlation between virulence of the organism and the presence of plasmid and amplification of Vir gene.
Page(s): 37-40
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(1) [January 2004]

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