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Title: Detection of virulence in Indian isolates of <i style="">Salmonella </i>by polymerase chain reaction <i style=""> </i>
Authors: Saxena, M K
Singh, V P
Lackchura, B D
Saxena, Anjani
Sharma, Bhaskar
Keywords: <i style="">Salmonella</i>
polymerase chain reaction (PCR)
<i style="">Vir</i> gene
Issue Date: Jan-2004
Publisher: CSIR
Abstract: <smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place" downloadurl=""> Virulence in <i style="">Salmonella</i> is reported to be caused by large plasmids of 60-100 kb. In the present study, 24 isolates of four <i style="">Salmonella</i> serovars (<i style="">S</i>. Dublin, <i style="">S</i>. Abortusequi, <i style="">S</i>. Bareilly and <i style="">S</i>. Choleraesuis) were studied for their <i style="">in vivo</i> virulence, plasmid profiling and virulence (<i style="">Vir</i>) gene detection by PCR. Nineteen isolates were found to be virulent inducing different degrees of mortality in mice. In 5 non-virulent isolates, the large plasmid was absent and there was also no amplification of <i style="">Vir</i> gene by PCR. It clearly indicates a positive correlation between virulence of the organism and the presence of plasmid and amplification of <i style="">Vir</i> gene. </smarttagtype>
Description: 37-40
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.03(1) [January 2004]

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