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Title: Embryo rescue: A tool to overcome incompatible interspecific hybridization in Gossypium Linn. - A review
Authors: Mehetre, S S
Aher, A R
Keywords: Gossypium spp.
embryo rescue
interspecific
Issue Date: Jan-2004
Publisher: CSIR
Abstract: Successful introgression of desirable characters from certain species could not be achieved because of different incompatibility reasons. Such incompatibility can be overcome by ovulo-embryo culture. From the literature reviewed so far, it appears that the technique involving excision of 10 to 15-day-old (after pollination) embryos and their subsequent culture on different combinations of BT and MS media was successful in obtaining difficult interspecific hybrids in Gossypium spp. BT and MS media supplemented with phytohormones, casein hydrolysate and IAA were more effective for culturing interspecific hybrid embryos. Other factors, like age and genotype of embryo; basal media, temperature and photoperiod during culturing; and hardening of plants are also important in determining the success of the interspecific embryo culture in Gossypium spp.
Page(s): 29-36
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.03(1) [January 2004]

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