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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.46 [2009] >
IJBB Vol.46(4) [August 2009] >
| Title: | Purification and characterization of bacteriocin produced by strain of Lactobacillus brevis MTCC 7539 |
| Authors: | Gautam, Neha
Sharma, Nivedita |
| Keywords: | Bacteriocin
Lactibacillus brevis |
| Issue Date: | Aug-2009 |
| Publisher: | CSIR |
| Abstract: | Bacteriocin, an antimicrobial agent having potential for food biopreservation was purified from Lactobacillus brevis (a safe food-grade bacteria isolated from Vari Kandal, a traditional fermented food of Himachal Pradesh by adopting a novel repeated washing method. Its purity was confirmed by SDS-PAGE and Native-PAGE. The relative molecular mass of bacteriocin was 93.74 kD, while specific activity and recovery were 35.52 folds and 17.13%, respectively. It showed high thermal stability and was active over wide range of pH and exhibited sensitivity to trypsin. |
| Page(s): | 337-341 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.46(4) [August 2009]
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