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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.46 [2009] >
IJBB Vol.46(4) [August 2009] >
| Title: | Effect of nickel on root growth and the kinetics of metal ions transport in onion (Allium cepa) root |
| Authors: | Akbaş, Halide
Dane, Feruzan
Meriç, Çiler |
| Keywords: | Allium cepa L
Atomic absorption spectrometry
Flame photometer
Nickel
Phytotoxic effect |
| Issue Date: | Aug-2009 |
| Publisher: | CSIR |
| Abstract: | The effect of different concentrations of nickel nitrate (0.25, 0.50, 1.00 and 2.00 mM) uptake by the roots, on root growth of onion (Allium cepa) and the transport of Ni2+, Fe2+, Mn2+, Zn2+, K+, Na+ and H+ ions were investigated spectrophotometrically. The uptake of Ni2+, Fe2+, Mn2+ and Zn2+ was monitored by flame atomic absorption spectrometry with a 24-h period for 7 days and the amounts of K+ and Na+ were determined in solutions by flame photometer. The mineral content of the solution, instead of the root material was measured. Ni2+ ions showed inhibitory effect on the root growth at all concentrations during the entire treatment. The EC50 (effective concentration that reduced root growth by 50%) was found at 0.25 mM Ni2+. No significant change in inhibitory effect was observed after at 0.50 mM Ni2+ concentrations. A large amount of Ni2+ was translocated into the roots. The kinetics of metal ion transport followed a pseudo-first order reaction in all metal ion concentrations. Ni2+, Zn2+ Fe2+ Mn2+ and H+ ions transferred together into plant, but Na+ and K+ ions transferred to the solution from the plant. The amount of H+ in the solution decreased at all Ni2+ concentrations. |
| Page(s): | 332-336 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.46(4) [August 2009]
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