NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.46 [2009] >
IJBB Vol.46(4) [August 2009] >

Title: Characterization of erythrosine B binding to bovine serum albumin and bilirubin displacement
Authors: Mathavan, Vinodaran M K
Boh, Boon Kim
Tayyab, Saad
Keywords: Absorption spectroscopy
Bilirubin displacement
Bovine serum albumin
Erythrosine B
Issue Date: Aug-2009
Publisher: CSIR
Abstract: The interaction of erythrosine B (ErB), a commonly used dye for coloring foods and drinks, with bovine serum albumin (BSA) was investigated both in the absence and presence of bilirubin (BR) using absorption and absorption difference spectroscopy. ErB binding to BSA was reflected from a significant red shift of 11 nm in the absorption maximum of ErB (527 nm) with the change in absorbance at λmax. Analysis of absorption difference spectroscopic titration results of BSA with increasing concentrations of ErB using Benesi-Hildebrand equation gave the association constant, K as 6.9 104 M-1. BR displacing action of ErB was revealed by a significant blue shift in the absorption maximum, accompanied by a decrease in absorbance difference at λmax in the difference spectrum of BR-BSA complex upon addition of increasing concentrations of ErB. This was further substantiated by fluorescence spectroscopy, as addition of increasing concentrations of ErB to BR-BSA complex caused a significant decrease in fluorescence at 510 nm. The results suggest that ErB binds to a site in the vicinity of BR binding site on BSA. Therefore, intake of ErB may increase the risk of hyperbilirubinemia in the healthy subjects.
Page(s): 325-331
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Source:IJBB Vol.46(4) [August 2009]

Files in This Item:

File Description SizeFormat
IJBB 46(4) 325-331.pdf338.87 kBAdobe PDFView/Open
 Current Page Visits: 1029 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 167018 since 01-Sep-2015  Last updated on 28-Jun-2016Webmaster: