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Indian Journal of Biochemistry and Biophysics (IJBB) >
IJBB Vol.46 [2009] >
IJBB Vol.46(4) [August 2009] >
| Title: | Properties of alkaline protease genetically engineered on cell surface of the yeast Yarrowia lipolytica |
| Authors: | Ni, Xiumei
Yue, Lixi
Li, Jing
Chi, Zhenming
Liu, Zhiqiang
Madzak, Catherine |
| Keywords: | Yeast surface display
Alkaline protease
Aureobasidium pullulans
Yarrowia lipolytica
Marine yeast |
| Issue Date: | Aug-2009 |
| Publisher: | CSIR |
| Abstract: | ALP2 gene encoding alkaline protease cloned from Aureobasidium pullulans HN2-3 was ligated into the surface display plasmid and expressed in the cells of the yeast Yarrowia lipolytica. The expressed alkaline protease was immobilized on the yeast cells. The activity of the immobilized enzyme with 6 His tag was found to be significantly higher than that of without 6 His tag. The immobilized enzyme showed lower optimal temperature and a lower affinity for azocasein than the free enzyme purified from A. pullulans HN2-3. The thermal stability of the immobilized enzyme enhanced and the pH stability decreased, compared to that of the free enzyme. |
| Page(s): | 294-298 |
| ISSN: | 0975-0959 (Online); 0301-1208 (Print) |
| Source: | IJBB Vol.46(4) [August 2009]
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