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Title: Properties of alkaline protease genetically engineered on cell surface of the yeast <i style="">Yarrowia lipolytica</i>
Authors: Ni, Xiumei
Yue, Lixi
Li, Jing
Chi, Zhenming
Liu, Zhiqiang
Madzak, Catherine
Keywords: Yeast surface display
Alkaline protease
<i style="">Aureobasidium pullulans</i>
<i style="">Yarrowia lipolytica</i>
Marine yeast
Issue Date: Aug-2009
Publisher: CSIR
Abstract: <i style="">ALP2</i> gene encoding alkaline protease cloned from <i style="">Aureobasidium pullulans </i>HN2-3 was ligated into the surface display plasmid and expressed in the cells of the yeast <i style="">Yarrowia lipolytica</i>. The expressed alkaline protease was immobilized on the yeast cells. The activity of the immobilized enzyme with 6<img src='/image/spc_char/cross.gif'> His tag was found to be significantly higher than that of without 6<img src='/image/spc_char/cross.gif'> His tag. The immobilized enzyme showed lower optimal temperature and a lower affinity for azocasein than the free enzyme purified from <i style="">A. pullulans </i>HN2-3. The thermal stability of the immobilized enzyme enhanced and the pH stability decreased, compared to that of the free enzyme.
Description: 294-298
ISSN: 0975-0959 (Online); 0301-1208 (Print)
Appears in Collections:IJBB Vol.46(4) [August 2009]

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