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Title: Quantification of phenolic compounds in Leucas mollissima Wall. ex Benth through HPTLC and validation of its antioxidant potential
Authors: Shukla, Pushpendra Kumar
Misra, Ankita
Srivastava, Akanksha
Srivastava, Sharad
Keywords: Catechin;HPTLC;Leucas mollissima;Quantification;Vanillic acid;Validation
Issue Date: Jul-2021
Publisher: NIScPR-CSIR, India
IPC Code: Int Cl.21: A61K 8/365, A61K 9/00, A61K 31/353, A61K 36/00, A61K 36/53
Abstract: The present study deals with quantification of phenolic compounds by a simple, rapid, sensitive and selective high-performance thin-layer chromatographic (HPTLC) method and evaluation of its traditional claims. A chromatographic separation was performed by using a combination of toluene: ethyl acetate: formic acid (70:30:10, v/v/v) as a mobile phase. A densitometric absorption mode has been used for the estimation of catechin and vanillic acid by comparing the peak area against the standard at wavelength 220 nm. The amount of catechin and vanillic acid was found to be 0.15% and 0.45% dry weight basis. The developed method was validated and found to be specific, linear, precise and accurate as per the International Conference on Harmonization guidelines. Antioxidant potential was evaluated by five different models having variable mechanisms of action viz., total phenolic and flavonoid content, reducing power assay, DPPH assay, deoxyribose assay and total antioxidant potential. In vitro potential data reveals that the species has significant potential and may be used as an alternative plant in future for the treatment of diabetic condition. However, identified bioactive compounds (catechin and vanillic acid) may be used as a reference tool for proper recognition and confirmation of right plant material and monitoring of batch-to-batch consistency of finished herbal products using Leucas mollissima as an ingredient.
Page(s): 694-699
ISSN: 0975-1068 (Online); 0972-5938 (Print)
Appears in Collections:IJTK Vol.20(3) [July 2021]

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