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|Title:||Biofabrication of Silver Oxide Nanoparticles (SO-NP) by autolysate of Pseudomonas mendocina PM1, and assessment of its antimicrobial/antibiofilm potential|
Babele, Piyoosh Kumar
|Keywords:||Acalypha indica;Glycine max;Pseudomonas aeruginosa;Pseudomonas mendocina PM1;Rhodobacter sphaeroides|
|Abstract:||Silver oxide Nanoparticles (SO-NP) exhibit excellent light absorbing, semi conducting properties and hence are employed in a wide range of applications such as catalyst, biosensors, and in fuel cells. Green synthesis of nanoparticles using different microorganisms is widely accepted since this method is in expensive and eco-friendly. Nanoparticles synthesized by this route are smaller in size, highly stable, show high reactivity and stability. In this context, biofabrication of Silver Oxide Nanoparticles (SO-NP) by autolysate of Pseudomonas mendocina PM1 has been evaluated. Synthesis of SO-NP was observed, when autolysate of P. mendocina PM1 was incubated with 0.5 mM AgNO3 in dark for 24 h. Synthesis of SO-NP was confirmed by UV-Vis analysis. SO-NP was further confirmed by Transmission Electron Microscopy (TEM) and X-ray Diffraction (XRD) which confirmed presence of SO-NP. XRD revealed that SO-NP were of the type Ag3O4. FTIR analysis indicated that peptides were involved in the reduction and stability of SO-NP. SO-NP’s showed potent anti-microbial/ anti-biofilm activity against common pathogenic/non-pathogenic bacteria. This is the first report of synthesis of SO-NP by P. mendocina PM1.|
|ISSN:||0975-0959 (Online); 0301-1208 (Print)|
|Appears in Collections:||IJBB Vol.58(4) [August 2021]|
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