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Title: Influence of fermentation conditions on levan production by Zymomonas mobilis CT2
Authors: Senthilkumar, V
Gunasekaran, P
Keywords: Zymomonas mobilis;levansucrase;levan;sucrase;fermentation
Issue Date: Oct-2005
Publisher: CSIR
IPC Code: Int. Cl.7 C12N1/16; C12R1:01; C12N9/24
Abstract: Zymomonas mobilis produces two extracellular sucrases namely levansucrase (sac B) and sucrase (sac C). A mutant strain of Z. mobilis CT2 defective in sucrase sac C, constructed earlier by gene disruption1, produced higher levels of levan (27.2 g L-1) than the parent strain B14023 (15.4 g L-1) from 200 g L-1 of sucrose at 25°C and pH 5.0. Increasing fermentation temperature from 25 to 35°C enhanced ethanol concentration from 17.8 to 46.4 g L-1 due to increased rate of sucrose hydrolysis but decreased the transfructosylation activity from 165 to 56 U mL-1. Addition of glucose or fructose to the fermentation medium considerably reduced the levan production due to the inhibition of levansucrase activity.
Page(s): 491-496
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(4) [October 2005]

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