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Title: Influence of fermentation conditions on levan production by <i style="">Zymomonas mobilis</i> CT2
Authors: Senthilkumar, V
Gunasekaran, P
Keywords: <i style="">Zymomonas mobilis</i>
Issue Date: Oct-2005
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> C12N1/16; C12R1:01; C12N9/24
Abstract: <i style="">Zymomonas mobilis</i> produces two extracellular sucrases namely levansucrase (<i style="">sac </i>B) and sucrase (<i style="">sac </i>C). A mutant strain of <i style="">Z. mobilis</i> CT2 defective in sucrase <i style="">sac</i> C, constructed earlier by gene disruption<sup>1</sup>, produced higher levels of levan (27.2 g L<sup>-1</sup>) than the parent strain B14023 (15.4 g L<sup>-1</sup>) from 200 g L<sup>-1</sup> of sucrose at 25°C and <i style="">p</i>H 5.0. Increasing fermentation temperature from 25 to 35°C enhanced ethanol concentration from 17.8 to 46.4 g L<sup>-1</sup> due to increased rate of sucrose hydrolysis but decreased the transfructosylation activity from 165 to 56 U mL<sup>-1</sup>. Addition of glucose or fructose to the fermentation medium considerably reduced the levan production due to the inhibition of levansucrase activity.
Description: 491-496
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(4) [October 2005]

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