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Title: Production of microbial iron chelators (siderophores) by fluorescent Pseudomonads
Authors: Sayyed, R Z
Badgujar, M D
Sonawane, H M
Mhaske, M M
Chincholkar, S B
Keywords: iron
optimization
Pseudomonads
siderophores
wheat growth
Issue Date: Oct-2005
Publisher: CSIR
IPC CodeInt.Cl.7 C08G59/70; C12R1:39,1:40
Abstract: Two fluorescent pseudomonads, Pseudomonas fluorescens NCIM 5096 and P. putida NCIM 2847 produced maximum yield of hydroxamate type of siderophore (87 & 83% units, respectively) in modified succinic acid medium (SM). (NH4)2SO4 and amino acids were found to stimulate bacterial growth as well as siderophore production. However, optimum siderophore yield was obtained with urea. Increase in iron concentration up to 100 µM favoured growth but drastically affected siderophore production in both the strains. Threshold level of iron (FeCl3), which repressed siderophore production in both the strains, was 30 µM. Sunflower oil proved to be suitable and cost effective defoaming agent for siderphore production in bioreactors. The results of shake flask level were found reproducible at scaled up conditions in bioreactors. Moreover, P. fluorescens NCIM 5096 inoculation enhanced seed germination, root length and shoot length of wheat (Triticum aestivum) under pot culture conditions.
Page(s): 484-490
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(4) [October 2005]

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