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Title: Variation in the volatile constituents of wild and in vitro propagated Tanacetum sinaicum Del. ex DC through GC-MS chemical fingerprint
Authors: Adel, Rasha
Abdel-Ghani, Afaf E
Abouelenein, Doaa D
El-Dahmy, Samih I
Keywords: GC-MS profile;Micropropagation;n-Hexane extract;Tanacetum sinaicum;Tissue culture
Issue Date: Jun-2021
Publisher: NIScPR-CSIR, India
IPC Code: Int. cl. (2015.01)-A01H, A01H 4/00
Abstract: Tanacetum sinaicum (Asteraceae) is a rare perennial herb growing wild in the mountains of Southern Sinai (Egypt). It is a medicinal and endangered plant. So, this work aimed to develop an in vitro propagation method for the conservation of this highly threatened plant. Sterile seedlings were used as a source of explants which were cultivated on Murashige and Skoog (M&S) media supplemented with different combinations of growth regulators for callus formation and induction of shoots and roots. M&S media with 1 mg/L kinetin (Kn) showed direct shoot induction. For root induction, excised shoots were transferred to M&S medium supplemented with 1 mg/L naphthalene acetic acid (NAA). Moreover, n-hexane extracts of wild and in vitro propagated plants were analyzed for their volatile constituents by gas chromatography-mass spectrometry (GC-MS) which resulted in the identification of 38 and 27 constituents, accounting for 87.75 and 75.51 % of their total composition; respectively.
Page(s): 238-246
ISSN: 0976-0512 (Online); 0976-0504 (Print)
Appears in Collections:IJNPR Vol.12(2) [June 2021]

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