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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(4) [October 2005] >
| Title: | Preparation of a reusable enzyme strip for determination of serum cholesterol |
| Authors: | Bhatia, Deepak
Suman
Pundir, C S |
| Keywords: | alkylamine glass
cholesterol
cholesterol esterase
cholesterol oxidase
enzyme strip
peroxidase
serum |
| Issue Date: | Oct-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl7 G01N33/535 |
| Abstract: | A reusable enzyme strip for the determination of cholesterol was prepared by co-immobilizing cholesterol esterase (CEase), cholesterol oxidase (COD) and peroxidase (POD) onto alkylamine glass beads affixed on a plastic strip by a non-reactive fixative. The co-immobilized enzymes retained 71.42% of initial activity of free enzymes with a conjugation yield of 11.0 mg g-1 beads and showed optimum activity at pH 6.6, when incubated at 32°C for 6 min. The strip was employed for direct determination of total cholesterol in serum. The minimum detection limit of the method was 2.14 mg dL-1 reaction mixture. The within batch and between batch coefficient of variation (CV) were <7.0% and <19.0%, respectively. The serum total cholesterol values as determined by the strip, were in good correlation (r=0.885) with those by commercial enzo-kit method employing free enzymes. The method is characterized by reuse of co-immobilized enzymes with great ease and unaffected by various serum substances. |
| Page(s): | 471-475 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(4) [October 2005]
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