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|Title:||Preparation of a reusable enzyme strip for determination of serum cholesterol|
Pundir, C S
|Series/Report no.:||Int. Cl<sup>7</sup> G01N33/535|
|Abstract:||A reusable enzyme strip for the determination of cholesterol was prepared by co-immobilizing cholesterol esterase (CEase), cholesterol oxidase (COD) and peroxidase (POD) onto alkylamine glass beads affixed on a plastic strip by a non-reactive fixative. The co-immobilized enzymes retained 71.42% of initial activity of free enzymes with a conjugation yield of 11.0 mg g<sup>-1</sup> beads and showed optimum activity at <i style="">p</i>H 6.6, when incubated at 32°C for 6 min. The strip was employed for direct determination of total cholesterol in serum. The minimum detection limit of the method was 2.14 mg dL<sup>-1</sup> reaction mixture. The within batch and between batch coefficient of variation (CV) were <7.0% and <19.0%, respectively. The serum total cholesterol values as determined by the strip, were in good correlation (<i style="">r</i>=0.885) with those by commercial enzo-kit method employing free enzymes. The method is characterized by reuse of co-immobilized enzymes with great ease and unaffected by various serum substances.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.04(4) [October 2005]|
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