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Title: Preparation of a reusable enzyme strip for determination of serum cholesterol
Authors: Bhatia, Deepak
Suman
Pundir, C S
Keywords: alkylamine glass
cholesterol
cholesterol esterase
cholesterol oxidase
enzyme strip
peroxidase
serum
Issue Date: Oct-2005
Publisher: CSIR
IPC CodeInt. Cl7 G01N33/535
Abstract: A reusable enzyme strip for the determination of cholesterol was prepared by co-immobilizing cholesterol esterase (CEase), cholesterol oxidase (COD) and peroxidase (POD) onto alkylamine glass beads af­fixed on a plastic strip by a non-reactive fixative. The co-immobilized enzymes retained 71.42% of initial activity of free enzymes with a conjugation yield of 11.0 mg g-1 beads and showed optimum activity at pH 6.6, when incubated at 32°C for 6 min. The strip was employed for direct determination of total cholesterol in serum. The minimum detection limit of the method was 2.14 mg dL-1 reaction mixture. The within batch and between batch coefficient of variation (CV) were <7.0% and <19.0%, respectively. The serum total cholesterol values as determined by the strip, were in good correlation (r=0.885) with those by commercial enzo-kit method employing free enzymes. The method is characterized by reuse of co-immobilized enzymes with great ease and unaffected by various serum substances.
Page(s): 471-475
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(4) [October 2005]

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