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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(4) [October 2005] >
| Title: | Micropropagation of Entada pursaetha DC-An endangered medicinal plant of Western Ghats |
| Authors: | Vidya, S M
Krishna, V
Manjunatha, B K
Shankarmurthy, K |
| Keywords: | Entada pursaetha
cotyledonary node
shoot organogenesis
micropropagation |
| Issue Date: | Oct-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A01H4/00, 5/04, 5/05 |
| Abstract: | A micropropagation protocol has been standardized for an endangered leguminous woody climber, Entada pursaetha using cotyledonary node explants. The synergetic effect of BAP (5 mg/L) with NAA (0.5 mg/L) induced a mean of 9.8±1.23 adventitious shoots from the cotyledonary node. The frequency of shoot production was highest (98.7%) in proximal transverse half of the cotyledon. The microshoots rooted well on MS half strength medium supplemented with 2 mg/L IBA. 70% of the hardened regenerants were acclimatized to the soil. |
| Page(s): | 561-564 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(4) [October 2005]
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