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Title:  Micropropagation of Entada pursaetha DC-An endangered medicinal plant of Western Ghats
Authors: Vidya, S M
Krishna, V
Manjunatha, B K
Shankarmurthy, K
Keywords: Entada pursaetha;cotyledonary node;shoot organogenesis;micropropagation
Issue Date: Oct-2005
Publisher: CSIR
IPC Code: Int. Cl.7 A01H4/00, 5/04, 5/05
Abstract: A micropropagation protocol has been standardized for an endangered leguminous woody climber, Entada pursaetha using cotyledonary node explants. The synergetic effect of BAP (5 mg/L) with NAA (0.5 mg/L) induced a mean of 9.8±1.23 adventitious shoots from the cotyledonary node. The frequency of shoot production was highest (98.7%) in proximal transverse half of the cotyledon. The microshoots rooted well on MS half strength medium supplemented with 2 mg/L IBA. 70% of the hardened regenerants were acclimatized to the soil.
Page(s): 561-564
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(4) [October 2005]

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