NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(4) [October 2005] >

Title: Shoot regeneration from immature cotyledonary nodes in black gram [Vigna mungo (L.) Hepper]
Authors: Muruganantham, M
Ganapathi, A
Amutha, S
Vengadesan, G
Selvaraj, N
Keywords: axillary meristem
black gram
immature cotyledonary node
Vigna mungo
Issue Date: Oct-2005
Publisher: CSIR
IPC CodeInt. Cl.7 A01H4/00, 5/04
Abstract: Eighteen-day-old immature cotyledonary node explants (18 d after anthesis) of black gram produced multiple shoots in MS salts+B5 vitamins containing medium in the presence of BA (1.0 mg/L), TDZ (0.1 mg/L) and AdS (15 mg/L). Maximum shoot proliferation (28 shoot/explant) occurred at the end of second subculture after 45 d. Periodic excision of regenerated shoots from explants increased shoot regeneration efficiency during subculture. The combination of TDZ and AdS with BA significantly increased shoot proliferation. Elongation and rooting were performed in GA3 (0.6 mg/L) and IBA (1.0 mg/L) containing media, respectively. The in vitro raised plantlets were acclimatized in green house and successfully transplanted to the field with a survival rate of 60%.
Page(s): 551-555
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(4) [October 2005]

Files in This Item:

File Description SizeFormat
IJBT 4(4) 551-555.pdf262.59 kBAdobe PDFView/Open
 Current Page Visits: 978 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 169376 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: