Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5766
Title: Shoot regeneration from immature cotyledonary nodes in black gram [<i style="">Vigna</i> <i style="">mungo</i> (L.) Hepper]
Authors: Muruganantham, M
Ganapathi, A
Amutha, S
Vengadesan, G
Selvaraj, N
Keywords: axillary meristem
black gram
immature cotyledonary node
subculture
<i style="">Vigna</i> <i style="">mungo</i>
Issue Date: Oct-2005
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A01H4/00, 5/04
Abstract: Eighteen-day-old immature cotyledonary node explants (18 d after anthesis) of black gram produced multiple shoots in MS salts+B5 vitamins containing medium in the presence of BA (1.0 mg/L), TDZ (0.1 mg/L) and AdS (15 mg/L). Maximum shoot proliferation (28 shoot/explant) occurred at the end of second subculture after 45 d. Periodic excision of regenerated shoots from explants increased shoot regeneration efficiency during subculture. The combination of TDZ and AdS with BA significantly increased shoot proliferation. Elongation and rooting were performed in GA<sub>3</sub> (0.6 mg/L) and IBA (1.0 mg/L) containing media, respectively. The <i style="">in</i> <i style="">vitro</i> raised plantlets were acclimatized in green house and successfully transplanted to the field with a survival rate of 60%.
Description: 551-555
URI: http://hdl.handle.net/123456789/5766
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(4) [October 2005]

Files in This Item:
File Description SizeFormat 
IJBT 4(4) 551-555.pdf262.59 kBAdobe PDFView/Open


Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.