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Title: Efficient plant regeneration from encapsulated somatic embryos of Hyoscyamus muticus L.
Authors: Pandey, Avantika
Chand, Suresh
Keywords: cotyledonary leaf
Hyoscyamus muticus
plant regeneration
somatic embryos
synthetic seed
Issue Date: Oct-2005
Publisher: CSIR
IPC CodeInt. Cl.7 A01H4/00, 5/04, 5/06
Abstract: A protocol has been developed for plant regeneration from encapsulated somatic embryos of Hyoscyamus muticus L. Somatic embryogenesis was achieved in callus cultures derived from cotyledonary leaf pieces on MS (1962) medium enriched with NAA (0.26–2.64 M) and BAP (2.2 M). For synthetic seed production and subsequent conversion into plantlets, somatic embryos were encapsulated using 3% sodium alginate and 75 mM CaCl2 as gel matrix. Maximum conversion frequency of 69% was noted from encapsulated somatic embryos cultured on MS medium without plant growth regulators. Plantlets with well developed shoots and roots were transferred to pots containing autoclaved mixture of peat moss, compost and soil (1:1:1). The encapsulated embryos could be stored up to 60 days at 4oC. There was negligible mortality of complete plants while they were being transferred from artificial soil to natural soil and finally to the open field.
Page(s): 546-550
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(4) [October 2005]

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