Please use this identifier to cite or link to this item: http://nopr.niscair.res.in/handle/123456789/5765
Title: Efficient plant regeneration from encapsulated somatic embryos of <i style="">Hyoscyamus muticus</i> L.
Authors: Pandey, Avantika
Chand, Suresh
Keywords: cotyledonary leaf
<i style="">Hyoscyamus muticus</i>
plant regeneration
somatic embryos
synthetic seed
Issue Date: Oct-2005
Publisher: CSIR
Series/Report no.: Int. Cl.<sup>7</sup> A01H4/00, 5/04, 5/06
Abstract: A protocol has been developed for plant regeneration from encapsulated somatic embryos of <i style="">Hyoscyamus muticus</i> L. Somatic embryogenesis was achieved in callus cultures derived from cotyledonary leaf pieces on MS (1962) medium enriched with NAA (0.26–2.64 <img src='/image/spc_char/micro.gif'> <i style="">M</i>) and BAP (2.2 <img src='/image/spc_char/micro.gif'> <i style="">M</i>). For synthetic seed production and subsequent conversion into plantlets, somatic embryos were encapsulated using 3% sodium alginate and 75 m<i style="">M</i> CaCl<sub>2</sub> as gel matrix. Maximum conversion frequency of 69% was noted from encapsulated somatic embryos cultured on MS medium without plant growth regulators. Plantlets with well developed shoots and roots were transferred to pots containing autoclaved mixture of peat moss, compost and soil (1:1:1). The encapsulated embryos could be stored up to 60 days at 4<sup>o</sup>C. There was negligible mortality of complete plants while they were being transferred from artificial soil to natural soil and finally to the open field.
Description: 546-550
URI: http://hdl.handle.net/123456789/5765
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(4) [October 2005]

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