NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(3) [July 2005] >

Title: Somatic embryogenesis and plantlet regeneration from cotyledon and leaf explants of Solanum surattense
Authors: Swamy, N Rama
Ugandhar, T
Praveen, M
Venkataiah, P
Rambabu, M
Upender, M
Subhash, K
Keywords: S. surattense
Indian solanum
somatic embryos
Issue Date: Jul-2005
Publisher: CSIR
IPC CodeInt. Cl.7 A01H 4/00, 5/10, 5/12
Abstract: Somatic embryogenesis and plant regeneration from the cotyledon and leaf explants of Indian Solanum (Solanum surattense Burm. f.), a medicinally important plant, is reported. Embryogenic callus was induced from cotyledon and leaf explants on Murashige and Skoog’s medium fortified with 0.5-8.0 mg/L -naphthalene acetic acid (NAA) + 0.5 mg/L N6-benzylaminopurine (BAP). High frequency of somatic embryo formation was found at 6.0 mg/L NAA + 0.5 mg/L BAP and 4.0 mg/L NAA+ 0.5 mg/L BAP in cotyledon and leaf explants, respectively. Secondary somatic embryogenesis was also observed when primary somatic embryos were subcultured on the same somatic embryo induction medium. Well-developed cotyledonary stage embryos were germinated on MS medium supplemented with 0.5 mg/L indole-3-acetic acid (IAA) +1.0-8.0 mg/L BAP. Maximum percentage (71.2%) of somatic embryo germination and plantlet formation was found at 0.5 mg/L IAA+2.0 mg/L BAP, but they didn’t germinate on ½ MSO and MSO media. The post transplantation survival rate of plants was 65-70%. Plants and flowers formed were morphologically similar to mother plants. The present protocol can be used for genetic transformation experiments in S. surattense.
Page(s): 414-418
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(3) [July 2005]

Files in This Item:

File Description SizeFormat
IJBT 4(3) 414-418.pdf181.54 kBAdobe PDFView/Open
 Current Page Visits: 997 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 165412 since 01-Sep-2015  Last updated on 27-Jun-2016Webmaster: