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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(3) [July 2005] >
| Title: | Somatic embryogenesis and plantlet regeneration from cotyledon and leaf explants of Solanum surattense |
| Authors: | Swamy, N Rama
Ugandhar, T
Praveen, M
Venkataiah, P
Rambabu, M
Upender, M
Subhash, K |
| Keywords: | S. surattense
Indian solanum
somatic embryos
regeneration |
| Issue Date: | Jul-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A01H 4/00, 5/10, 5/12 |
| Abstract: | Somatic embryogenesis and plant regeneration from the cotyledon and leaf explants of Indian Solanum (Solanum surattense Burm. f.), a medicinally important plant, is reported. Embryogenic callus was induced from cotyledon and leaf explants on Murashige and Skoog’s medium fortified with 0.5-8.0 mg/L  -naphthalene acetic acid (NAA) + 0.5 mg/L N6-benzylaminopurine (BAP). High frequency of somatic embryo formation was found at 6.0 mg/L NAA + 0.5 mg/L BAP and 4.0 mg/L NAA+ 0.5 mg/L BAP in cotyledon and leaf explants, respectively. Secondary somatic embryogenesis was also observed when primary somatic embryos were subcultured on the same somatic embryo induction medium. Well-developed cotyledonary stage embryos were germinated on MS medium supplemented with 0.5 mg/L indole-3-acetic acid (IAA) +1.0-8.0 mg/L BAP. Maximum percentage (71.2%) of somatic embryo germination and plantlet formation was found at 0.5 mg/L IAA+2.0 mg/L BAP, but they didn’t germinate on ½ MSO and MSO media. The post transplantation survival rate of plants was 65-70%. Plants and flowers formed were morphologically similar to mother plants. The present protocol can be used for genetic transformation experiments in S. surattense. |
| Page(s): | 414-418 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(3) [July 2005]
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