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Title: Plant regeneration from tissue culture of Chloris virgata: A salt-tolerant desert grass
Authors: Dagla, Harchand R
Shekhawat, N S
Keywords: Chloris virgata
desert grass
salt tolerant
plantlet regeneration
Issue Date: Jul-2005
Publisher: CSIR
IPC CodeInt. Cl.7 A01H4/00, 5/10
Abstract: A tissue culture protocol for regeneration of salt-tolerant grass Chloris virgata Sw. is reported. Scutellum and axis of activated embryo produced callus on Murashige & Skoog (MS, 1962) medium with 2.0-45.0 M of 2, 4-dichlorophenoxyaceticacid (2,4-D). The explants produced two types of callus. Type-I produced on MS + 2.0-9.0 M 2, 4-D was amorphous, translucent and white; while Type-II produced on 22.0-45.0 M 2,4-D was creamy, compact and globular. The latter exhibited competence to regenerate into plants. These cultures were amplified on MS + 22.0 M 2,4-D. The regenerative cultures, on transfer to full, half and one-fourth strengths of growth regulator-free MS or full MS + 2.0 to 8.0 M benzyladenine (BAP) differentiated into plantlets. The highest regeneration potential (6-7 plantlets per inoculation) was achieved on full-strength growth regulator-free MS medium with 50 mg L-1 ascorbic acid and 25 mg L-1 each of adenine sulphate, arginine and citric acid. The tissue culture raised plants were hardened in a greenhouse with controlled environmental conditions. Ninety per cent of the regenerants survived during acclimatization. A total of 504 acclimatized plants were transferred to polybags and in field. Of these 80% survived in the polybags and 70% in the field.
Page(s): 400-403
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(3) [July 2005]

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