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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(3) [July 2005] >
| Title: | Partial purification and characterization of  -amylase produced by Aspergillus oryzae using spent-brewing grains |
| Authors: | Patel, Anil Kumar
Nampoothiri, K Madhavan
Ramachandran, Sumitra
Szakacs, George
Pandey, Ashok |
| Keywords: | -Amylase
Aspergillus oryzae
solid-state fermentation
spent brewing grains |
| Issue Date: | Jul-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A01N63/02; C12N9/30; C12R1: 69 |
| Abstract: | Solid-state fermentation (SSF) was carried out to produce -amylase from Aspergillus oryzae (IFO 30103) using spent brewing grains (SBG) as substrate. A maximum of 11296 U/gds amylase activity was obtained after 48 h of fermentation. The extracted enzyme was subjected to partial purification by ammonium sulphate fractionation. Maximum specific activity was obtained with 40-70% fraction. SDS-PAGE of the corresponding sample revealed an approximate 66 kDa band, which was confirmed by activity staining as -amylase. It was optimally active at pH 5 and 50°C by using 1% starch as substrate concentration. The partially purified -amylase loses activity rapidly above 50oC but it can be retained in the presence of Ca2+. Presence of Mn2+ and Fe2+ enhanced the enzyme activity and is almost doubled in presence of Mn2+. However, in the presence of Hg2+ and Cu2+, the activity is reduced. Hg2+ reduced the enzyme activity by half. |
| Page(s): | 336-341 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(3) [July 2005]
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