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Title: Microbial Bioagents: Economic multiplication and management of fungal-nematode complex on cumin
Authors: Sharma, Nidhi
Trivedi, P C
Keywords: biocontrol
Meloidogyne incognita
Fusarium oxysporum f. sp. cumini
Trichoderma harzianum
T. viride
T. hamatum
Bacillus subtilis
Pseudomonas fluorescens
Issue Date: Jul-2005
Publisher: CSIR
IPC CodeInt. Cl.7 A01N 63/04
Abstract: Mass-scale multiplication was done on cheaper substrates for application of bioagents in the management of plant-parasitic nematodes and fungal pathogens. The bioagents were isolated from the local field soils. Out of the 13 isolated fungi, most of the isolates of Trichoderma spp. that were found antagonistic to Fusarium oxysporum f. sp. cumini in dual culture technique, were mass multiplied on cheaper agrowastes. Suitability of 6 substrates was screened and tea waste was found to be best followed by wheat bran and sorghum straw. Trichoderma harzianum (T5) had the ma´imum spore load per gram (SLPG) value on tea waste followed by T. hamatum (T16) on wheat bran. Three isolates of bacteria viz. Bacillus subtitlis, Pseudomonas fluorescens and Rhizobium spp. were multiplied on nutrient broth, King’s B broth and yeast e´tract mannitol broth, respectively.
Page(s): 419-421
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(3) [July 2005]

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