NISCAIR Online Periodicals Repository

Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(3) [July 2005] >

Title: Micropropagation of Adhatoda vasica Nees–A woody medicinal plant by shoot tip culture
Authors: Nath, Sangeeta
Buragohain, Alak K
Keywords: thiadiazuron
Adhatoda vasica
phenolic exudates
shoot tip
Issue Date: Jul-2005
Publisher: CSIR
IPC CodeInt. Cl.7 A01H 4/00, 5.04
Abstract: Exudation was overcome during shoot tip (1 cm) culture of Adhatoda vasica by modifying the pH of the media and by lowering the concentrations of NH4+ and K+ ions. Though shoot formation occurred in media supplemented with BA (2 mg L-1), 96.67% of the shoots developed callus at the cut basal end and the explants turned brown and necrotic due to the phenolic exudates released into the medium. Development of callus tissue and browning was eliminated by culturing the shoot tip explants on primary MS medium supplemented with thiadiazuron (0.30 mg L-1) and coconut milk (15%) which also promoted development of shoots but with stunted curled leaves. Shoots (5.2) with healthy leaves were obtained when these shoots were transferred to a secondary MS medium supplemented with 0.5 mgL-1 BA. Incorporation of coconut milk (15%) in the secondary MS medium had a growth promontory effect. High frequency rooting (9.33) was recorded in MS basal medium. The rooted plantlets were hardened and established at 85% rate in pots.
Page(s): 396-399
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(3) [July 2005]

Files in This Item:

File Description SizeFormat
IJBT 4(3) 396-399.pdf105.74 kBAdobe PDFView/Open
 Current Page Visits: 1198 
Recommend this item


Online Submission of Articles |  NISCAIR Website |  National Knowledge Resources Consortium |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2015 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 170985 since 01-Sep-2015  Last updated on 30-Jun-2016Webmaster: