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IJBT Vol.04(3) [July 2005] >


Title: FISH and GISH: Modern cytogenetic techniques
Authors: Devi, J
Ko, J M
Seo, B B
Keywords: FISH
GISH
in situ hybridization
rDNA
physical map
probe labelling
Issue Date: Jul-2005
Publisher: CSIR
IPC CodeInt. Cl.7 A01H1/00
Abstract: In recent years, advances in the molecular cytogenetic technique of fluorescence in situ hybridization (FISH), which enables the direct chromosomal localization of labelled DNA probes and genomic in situ hybridization (GISH), which determines the inter-species distribution of repeated sequences have enabled a resurgence of cytogenetic analysis in plant genome research and molecular breeding. Practical applications of these techniques in chromosome mapping, genome analysis, determination of phylogenetic relationship, detection of chromosomal aberrations and alien chromatin in plant breeding programmes, study of chromosome organization at interphase nuclei and analysis of somaclonal variations in tissue culture have been presented.
Page(s): 307-315
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(3) [July 2005]

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