NISCAIR Online Periodicals Repository

NISCAIR ONLINE PERIODICALS REPOSITORY (NOPR)  >
NISCAIR PUBLICATIONS >
Research Journals >
Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(2) [April 2005] >


Title: Lipase from Pseudomonas aeruginosa MTCC 2488: Partial purification, characterization and calcium dependent thermostability
Authors: Kukreja, Vandana
Bera, M B
Keywords: calcium dependent thermostability
lipase
lipase activity
P aeruginosa
Tween-20
Issue Date: Apr-2005
Publisher: CSIR
IPC CodeInt. Cl.7 A01N63/02; C12N9/16, 9/20; C12R1: 385
Abstract: Psuedomonas aeruginosa MTCC 2488 produced lipase on Rhodamine B agar plates containing olive oil. Extra-cellular lipase activity was analyzed spectrophotometrically using Tween-20 as well as olive oil as substrate. The semipurified enzyme, precipitated by 30% saturated ammonium sulphate, showed 20.79 fold increase in specific activity (U/mg) and reduction in carbohydrate content to 1.7% as compared to the crude enzyme. The enzyme hydrolyzed Tween-20 and -40 better than Tween-60 and -80. Lipase has been found to be thermostable with maximum activity at 55-60oC but marked decrease was observed above this temperature. Ca2+ seemed to play an important role in the thermostability as 97% of enzyme activity was retained after 2 hr incubation at 65oC and 1hr incubation at 70oC in presence of 10 mM CaCl2. However, thermostability of the enzyme was decreased considerably in presence of 5 mM EDTA, confirming the enzyme to be a metalloprotein. Lipase has been found to be stable in presence of 30% acetone, methanol and ethanol. While, the enzyme activity was decreased by 30-50% in presence of n propanol, 2 propanol, n methyl propanol, isooctane and hexane. Further, 30% butanol resulted in ~65% decrease in the enzyme activity. Lipase has been found to be stable in presence of nonionic detergents, whereas anionic detergent, SDS completely inactivated the enzyme.
Page(s): 222-226
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Source:IJBT Vol.04(2) [April 2005]

Files in This Item:

File Description SizeFormat
IJBT 4(2) 222-226.pdf131.55 kBAdobe PDFView/Open
 Current Page Visits: 533 
Recommend this item

 

National Knowledge Resources Consortium |  NISCAIR Website |  Contact us |  Feedback

Disclaimer: NISCAIR assumes no responsibility for the statements and opinions advanced by contributors. The editorial staff in its work of examining papers received for publication is helped, in an honorary capacity, by many distinguished engineers and scientists.

CC License Except where otherwise noted, the Articles on this site are licensed under Creative Commons License: CC Attribution-Noncommercial-No Derivative Works 2.5 India

Copyright © 2012 The Council of Scientific and Industrial Research, New Delhi. All rights reserved.

Powered by DSpace Copyright © 2002-2007 MIT and Hewlett-Packard | Compliant to OAI-PMH V 2.0

Home Page Total Visits: 510901 since 06-Feb-2009  Last updated on 11-Apr-2014Webmaster: nopr@niscair.res.in