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Title: <i>In vitro</i> clonal propagation of vulnerable medicinal plant, <i>Saraca</i><i> asoca </i>(Roxb.) De Wilde
Authors: Subbu, R Rama
Chandraprabha, A
Sevugaperumal, R
Keywords: <i>Saraca</i><i> asoca</i>
Clonal propagation
Nodal segments
Shoot tip explants
Growth regulators
Issue Date: Aug-2008
Publisher: CSIR
Series/Report no.: <b>Int. cl.<sup>8</sup> </b>- A01G 9/00, A61K 36/00<b></b>
Abstract: <i>In vitro</i> clonal propagation of Ashoka, <b><i>Saraca</i></b><b><i> asoca </i>(Roxb.)</b><b> De Wilde </b>plant was achieved using shoot tip, nodal and internodal explants. The explants were cultured on MS medium supplemented with different concentrations (0.5-2.0mg/l) of Benzyl amino purine (BAP), Kinetin (Kn) and 2, 4-Dichlorophenoxy acetic acid (2,4-D). The synergetic effect of BAP (0.5mg/l) induced a mean of 11.71±0.53 adventitious shoots from the nodal explants. The frequency of shoot organogenesis was highest (82%) in nodal explants treated with 0.5mg/l of BAP and callus were formed more on 2, 4-D. The micro-shoots rooted well on MS medium supplemented with 4.0mg/l of IBA. Hardened regenerants (40%) were acclimatized to the soil.
Description: 338-341
ISSN: 0975-1092 (Online); 0972-592X (Print)
Appears in Collections:NPR Vol.7(4) [July-August 2008]

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