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Title: Identification and characterization of polymorphic genic SSR markers between cultivated (Oryza sativa) and Indian wild rice (Oryza nivara)
Authors: Ngangkham, Umakanta
Katara, Jawahar Lal
Shanmugavadivel, P S
Yadav, Manoj Kumar
Yadav, Suresh
Devachandra, Nongthombam
Samantaray, Sanghamitra
Bose, Lotan Kumar
Keywords: Indian wild rice;Oryza nivara;High yielding indica rice;Oryza sativa;Gene introgression;Mapping population
Issue Date: Oct-2020
Publisher: NISCAIR-CSIR, India
Abstract: In the present study, we developed a set of 100 BC2F4 mapping lines derived from the cross between the Oryza nivara (AC100476) and high yielding indica rice, Lalat. Out of 410 RM markers used for polymorphism survey between the parental lines, we identified around 113 (28.9%) polymorphic rice microsatellite (RM) markers between the parental lines that were uniformly distributed among the 12 chromosomes except for few gaps on chromosome 1, 7 and 10. On the basis of motif length, the trinucleotide repeats-motif, (TTA)n was the longest with a maximum motif length of 177 nucleotides. Among the repeat motifs, di-nucleotide repeat-motifs was the most abundant (68.14%) with the motifs (AC/GT)n were the most abundant accounting 56.32% of the total dinucleotide repeat-motifs. Out of the 113 polymorphic RM markers, 56 (49.55%) were found to be genic markers and broadly distributed into three groups such as molecular, biological and cellular categories with 38%, 32% and 30% frequencies, respectively. The present finding would be useful for the identification and mapping of drought related traits and development of drought tolerant rice cultivars in rice breeding program.
Page(s): 299-310
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.19(4) [October 2020]

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