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Title: Antimicrobial activity of Nigella sativa Linn. seed oilagainst multi-drug resistant bacteria from clinical isolates
Authors: Salman, M T
Khan, R A
Shukla, I
Keywords: Nigella sativa;Black cumin;Antimicrobial activity;Essential oil;Antibiotic resistance;Clinical isolates
Issue Date: Feb-2008
Publisher: CSIR
IPC Code: Int. cl.8— A61K 36/00, A61P 31/04
Abstract: An alarming increase in bacterial strains resistant to existing antimicrobial agents demands a renewed effort to seek agents effective against pathogenic bacteria resistant to currentantimicrobials. Nigella sativa Linn. (Black cumin) essential oil was studied for anti bacterial activity against various clinical isolates of bacteria resistant to a number of antibiotics, in varying concentrations by Disc Agar diffusion technique using impregnated filter paper discs on inoculated Muellar Hinton agar plates. The oil showed pronounced dose dependant antibacterial activity which was more against Gram positive than Gram negative bacteria. Among Gram positive bacteria tested, Staphylococcus aureus, S. epidermidis, other coagulase –ve Staphylococci and Streptococcus pyogenes were sensitive to the oil and Enterococcus faecalis, Streptococcus agalactiae were resistant. Among Gram –ve bacteria tested, only Pseudomonas aeruginosa was sensitive to oil and rest (Acinetobacter baumannii, Citrobacter freundii, Klebsiella pneumoniae, Proteus mirabilis, P. vulgaris and Vibrio cholerae) were insensitive. Out of 144 strains tested, most of which were resistant to a number of antibiotics, 97 were inhibited by the oil of black cumin. To the best of our knowledge, the activity of essential oil against coagulase negative Staphylococci (except S. epidermidis) and Streptococcus pyogenes is being reported for the first time.
Page(s): 10-14
ISSN: 0975-1092 (Online); 0972-592X (Print)
Appears in Collections:NPR Vol.7(1) [January-February 2008]

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