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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(1) [January 2005] >
| Title: | Degradation of phorate by Azotobacter isolates |
| Authors: | Kadam, T A
Gangawane, L V |
| Keywords: | Azotobacter
N fixation
phorate
tolerance |
| Issue Date: | Jan-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 A 01 N 63/02 |
| Abstract: | Of 44 Azotobacter isolates from the soils of Maharashtra, 29 belonged to A. chroococcum and other were A. vinelandii. All A. chroococcum isolates were efficient in N fixation when tested by acetylene reduction test and also in cotton cv NHH-44. However, they showed variation to phorate tolerance. The maximum tolerance (150  g/ml) was shown by ACI-17, ACI-18 and ACI-28 isolates. These isolates also showed maximum phorate degradation. TLC and HPLC studies indicated the presence of O,O-diethyl S-{(ethyl sulphonyl) methyl} phosphorothioate, O,O-diethyl S-{(ethyl sulphinyl) methyl} phosphorothioate and diethyl phosphoric acid as degradation products. Plasmid analysis indicated that the genes for phorate degradation might be located on the main chromosome and not on the plasmid. |
| Page(s): | 153-155 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(1) [January 2005]
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