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|Title:||Variability and molecular diversity of wild sugarcane germplasm collected from low temperature regions Lohit and Changlang of Arunachal Pradesh|
Narayan, J. Ashwin
Mahadevaswamy, H. K.
Meena, Mintu Ram
|Keywords:||Molecular diversity;STMS marker;Saccharum spontaneum;Germplasm;Sugarcane|
|Abstract:||Saccharum spontaneum L. is a perennial grass representing the most genetically diversified species in Saccharum genus. It has the potential to withstand severe biotic/abiotic stresses and frequently used as donor of stress tolerant genes in sugarcane improvement program through gene introgression. In this study, the phenotypic variation and molecular diversity of forty nine S. spontaneum accessions collected from Lohit and Changlang regions of Arunachal Pradesh, North Eastern India were investigated for morphometric traits and polymorphic STMS marker. The phenotypic coefficient of variation showed ample variability for the traits viz., plant height (27.19%), stalk diameter (28.21%), single cane weight (48.97%), internode number (22.60%) and internode length (29.15%). Further, twenty nine sequence-tagged microsatellite site (STMS) markers generated 495 bands with an average of 14.06 polymorphic bands. The accessions specific bands in respect to specific marker combinations were identified. The Jaccard’s similarity coefficients among these accessions ranged from 0.42 to 0.78 with an average of 0.58 and clustering using unweighted pair group method of arithmetic-average (UPGMA) showed two major clusters with subclusters. Similarly polulation structure analysis based Bayesian approach grouped the individuals into two subpopulations, with alpha value of 0.112. The study shows that S. spontaneum accessions collected from Arunachal Pradesh is highly diverse, most of them will be harbouring the genes for cold tolerance and biomass. The set of markers which produced specific bands for the specific accessions identified in the study will help in identification of the particular accessions. The accessions studied are potential source for cold tolerance and high biomass, the results obtained in the present study will definitely help in planning and utilising them in sugarcane improvement programme.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.19(3) [July 2020]|
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|IJBT 19(3) 159-168.pdf||Main Article||612.13 kB||Adobe PDF||View/Open|
|IJBT 19(3) 159-168_Suppl Data.pdf||Supplementary Data||181.85 kB||Adobe PDF||View/Open|
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