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|Title:||In vivo genomic expression of luteinizing hormone receptors and steroidogenic-enzyme in follicles of crossbred cows|
|Keywords:||Ovarian granulosa cell;LH receptor;Steroidogenic enzyme;Indian crossbred cows|
|Abstract:||The present work was aimed at documenting the in vivo expression patterns of luteinizing hormone receptor (LHr) and cytochrome P450 aromatase enzyme (CYP19) genes in the granulosa cells (GC) of dominant follicles (DF) in Indian crossbred cows. Six crossbred cows were utilized for the study. Ultrasound guided in vivo follicular aspiration was performed on the specific days of the cycle viz., Day 0-oestrus (preovulatory follicle-POF); Day 6-wave I growing DF (GDF) and Day 8-wave I regressing DF (RDF). Twelve follicles in each category were aspirated and GCs were separated. Qualitative and semi-quantitative analysis of LHr and CYP-19 genes in GCs of all the three categories of the DFs were studied by conventional polymerase chain reaction (PCR) and densitometry analysis. PCR studies showed that mRNA expression for the LHr was enhanced in GDF comparable with that of POF, but significantly reduced in RDF. Analysis of relative expression levels revealed that the LHr expression was reduced by 1.5 folds in Day 8 RDF when compared to GDF. The expression levels of CYP19 gene in POF were greater than GDF and RDF respectively. Increased expression levels of these genes explained the ovulatory nature of POF when compared to wave I GDF.|
|ISSN:||0975-0967 (Online); 0972-5849 (Print)|
|Appears in Collections:||IJBT Vol.19(3) [July 2020]|
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