2,4-Dinitrophenyl hydrazone derivatives as potent alpha amylase inhibitors

Abstract

In our current study thirteen new 2,4-dinitrophenyl hydrazone derivatives <strong>1&ndash;13</strong> have been evaluated for alpha amylase activity. The molecular docking results indicate that compounds potentially bind in the catalytic site of the enzyme with excellent result. <em>Molecular Operating Environment</em> (MOE) software was used for docking study. 2,4-Dinitrophenyl hydrazone <strong>1-13</strong> have been obtained under reflux conditions by reacting dinitrophenyl hydrazine in methanol with different aromatic as well as aliphatic aldehydes in the presence of acetic acid act as a catalyst. The current results have shown that compounds <strong>5</strong> (IC₅₀=12.16<em>&micro;</em>g/mL), <strong>6</strong> (IC₅₀=15.03<em>&micro;</em>g/mL), and <strong>12</strong> (IC₅₀=16.42 <em>&micro;</em>g/mL) have been found to be the more potent alpha amylase inhibitors as compared to the standard acarbose (IC₅₀= 42.47<em>&micro;</em>g/mL). These compounds may provide better leads for alpha amylase inhibitor and further assessment of these compounds can be of great help in the discovery of new antidiabetic drugs.