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Indian Journal of Biotechnology (IJBT) >
IJBT Vol.04 [2005] >
IJBT Vol.04(1) [January 2005] >
| Title: | Preclinical studies for gene therapy of head and neck cancers using the HSV-tk/GCV strategy |
| Authors: | Wagle, Archana S
Joshi, Ganesh V
Naresh, Kikkeri N
Mulherkar, Rita |
| Keywords: | gene therapy
HSV-tk
xenograft
nude mice
ganciclovir
head and neck cancer |
| Issue Date: | Jan-2005 |
| Publisher: | CSIR |
| IPC Code: | Int. Cl.7 C 12 N 5/16, 15/12, 15/28 |
| Abstract: | The present study has used the suicide gene Herpes Simplex Virus–thymidine kinase (HSV-tk) and ganciclovir (GCV) strategy. A 2 kb sequence of HSV-tk was subcloned into the retroviral vector resulting in a recombinant vector, LTKSN, which was transfected into a packaging cell-line, PA317 and selected on G418. The highest expressing clone, PTK-16 was used for in vitro experiments. Intra-tumoural injections of PTK-16 followed by GCV treatment, in a HNSCC xenograft model in nude mice, showed a significant reduction in the viable tumour volume (p=0.009). These results will form the basis for future clinical trials in HNSCC. |
| Page(s): | 82-87 |
| ISSN: | 0975-0967 (Online); 0972-5849 (Print) |
| Source: | IJBT Vol.04(1) [January 2005]
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