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Title: Preclinical studies for gene therapy of head and neck cancers using the HSV-tk/GCV strategy
Authors: Wagle, Archana S
Joshi, Ganesh V
Naresh, Kikkeri N
Mulherkar, Rita
Keywords: gene therapy;HSV-tk;xenograft;nude mice;ganciclovir;head and neck cancer
Issue Date: Jan-2005
Publisher: CSIR
IPC Code: Int. Cl.7 C 12 N 5/16, 15/12, 15/28
Abstract: The present study has used the suicide gene Herpes Simplex Virus–thymidine kinase (HSV-tk) and ganciclovir (GCV) strategy. A 2 kb sequence of HSV-tk was subcloned into the retroviral vector resulting in a recombinant vector, LTKSN, which was transfected into a packaging cell-line, PA317 and selected on G418. The highest expressing clone, PTK-16 was used for in vitro experiments. Intra-tumoural injections of PTK-16 followed by GCV treatment, in a HNSCC xenograft model in nude mice, showed a significant reduction in the viable tumour volume (p=0.009). These results will form the basis for future clinical trials in HNSCC.
Page(s): 82-87
ISSN: 0975-0967 (Online); 0972-5849 (Print)
Appears in Collections:IJBT Vol.04(1) [January 2005]

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